Volume 23, Issue 1 (January 1978)

    Electrophoresis of Esterase D in Fresh Blood and in Bloodstains on Cellulose Acetate

    (Received 23 May 1977; accepted 15 June 1977)

    Published Online: January

    CODEN: JFSOAD

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    Abstract

    Esterase D (EsD) was first phenotyped by Hopkinson et al [1] using starch gel electrophoresis. Three phenotypes were described: 1-1, 1-2, and 2-2. Bender and Frank [2] detected a new EsD phenotype, which they named 3-1. Typing of EsD in bloodstains with starch gel has been reported by Parkin and Adams [3]. Esterase D isoenzymes have also been separated by electrophoresis with polyacrylamide gel, agarose gel, and cellulose acetate by Köster et al [4]. They observed poor results with cellulose acetate and discontinued its use. Since the results with phenotyping EsD on cellulose acetate in our laboratory are unambiguous, fast, reproducible, and economical, it is obvious that good results depend on the technology employed.


    Author Information:

    Grunbaum, BW
    Research biochemist and staff research associates, White Mountain Research Station, University of California, Berkeley, Calif.

    Del Re, B
    Research biochemist and staff research associates, White Mountain Research Station, University of California, Berkeley, Calif.

    Harmor, GC
    Research biochemist and staff research associates, White Mountain Research Station, University of California, Berkeley, Calif.

    Zajac, PL
    Criminalist, Criminalistics Laboratory, Pleasanton, Calif.


    Stock #: JFS10657J

    ISSN: 0022-1198

    DOI: 10.1520/JFS10657J

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    Title Electrophoresis of Esterase D in Fresh Blood and in Bloodstains on Cellulose Acetate
    Symposium , 0000-00-00
    Committee E30