Volume 23, Issue 1 (January 1978)
The Analysis of Meperidine and Normeperidine in Biological Specimens
This study centers on the analytical aspects of meperidine analysis in biological specimens. The major meperidine metabolite normeperidine is treated concomitantly throughout as it is considered important in the interpretation of results in medical examiner/coroner cases and in overdosed patients. Previous studies have presented methods for the gas chromatographic analysis of meperidine in blood, plasma, or urine [1–3]. Other reports have presented procedures for meperidine and normeperidine [4–7] but did not deal with tissue analysis. Kazyak  reported combined meperidine and normeperidine in blood, urine, and four tissues but did not analyze the two compounds separately. A gas chromatographic method for meperidine and normeperidine in blood, urine, bile, liver, and other tissues is presented here. The method is straightforward with no derivatizing reactions prior to gas chromatography (GC); analysis time for liver is about 1 h, and blood and urine can be analyzed in 30 min. Results from twelve medical examiner cases are presented and discussed.