Volume 22, Issue 2 (April 1977)

    The Determination of Isoantigenic Activity from Latent Fingerprints: Mixed Cell Agglutination Reaction in Forensic Serology

    (Received 29 March 1976; accepted 12 October 1976)

    Published Online: April

    CODEN: JFSOAD

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    Abstract

    In forensic serology, A-B-H blood group substances have been successfully detected by mixed agglutination and absorption-elution techniques. By modifying the methods of Kouvarik et al [1] and Davidsohn [2], Ishiyama et al [3] recently developed a new technique for mixed cell agglutination reaction (MCAR) as follows: 1. Specimens were affixed onto the adhesive side of cellophane tape and then placed on a microscope slide. 2. Anti-A and anti-B agglutinins and Ulex anti-H lectin were used for sensitization. 3. The specimens were thoroughly washed to remove nonreactive agglutinins. 4. A corresponding indicator cell aliquot was added (sandwich method). 5. The slide was reversed and immersed in saline so that the nonreactive cells would dissociate spontaneously. 6. The results were read microscopically.


    Author Information:

    Ishiyama, I
    Teikyo University School of Medicine, Tokyo,

    Ogawa, K
    Teikyo University School of Medicine, Tokyo,

    Orui, M
    Teikyo University School of Medicine, Tokyo,

    Kimura, T
    Teikyo University School of Medicine, Tokyo,


    Stock #: JFS10598J

    ISSN: 0022-1198

    DOI: 10.1520/JFS10598J

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    Title The Determination of Isoantigenic Activity from Latent Fingerprints: Mixed Cell Agglutination Reaction in Forensic Serology
    Symposium , 0000-00-00
    Committee E30