The Determination of Isoantigenic Activity from Latent Fingerprints: Mixed Cell Agglutination Reaction in Forensic Serology

    Volume 22, Issue 2 (April 1977)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 April 1977

    Page Count: 11


    Ishiyama, I
    Teikyo University School of Medicine, Tokyo,

    Ogawa, K
    Teikyo University School of Medicine, Tokyo,

    Orui, M
    Teikyo University School of Medicine, Tokyo,

    Kimura, T
    Teikyo University School of Medicine, Tokyo,

    (Received 29 March 1976; accepted 12 October 1976)

    Abstract

    In forensic serology, A-B-H blood group substances have been successfully detected by mixed agglutination and absorption-elution techniques. By modifying the methods of Kouvarik et al [1] and Davidsohn [2], Ishiyama et al [3] recently developed a new technique for mixed cell agglutination reaction (MCAR) as follows: 1. Specimens were affixed onto the adhesive side of cellophane tape and then placed on a microscope slide. 2. Anti-A and anti-B agglutinins and Ulex anti-H lectin were used for sensitization. 3. The specimens were thoroughly washed to remove nonreactive agglutinins. 4. A corresponding indicator cell aliquot was added (sandwich method). 5. The slide was reversed and immersed in saline so that the nonreactive cells would dissociate spontaneously. 6. The results were read microscopically.


    Paper ID: JFS10598J

    DOI: 10.1520/JFS10598J

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    Title The Determination of Isoantigenic Activity from Latent Fingerprints: Mixed Cell Agglutination Reaction in Forensic Serology
    Symposium , 0000-00-00
    Committee E30