Journal Published Online: 01 April 1977
Volume 22, Issue 2

The Determination of Isoantigenic Activity from Latent Fingerprints: Mixed Cell Agglutination Reaction in Forensic Serology

CODEN: JFSCAS

Abstract

In forensic serology, A-B-H blood group substances have been successfully detected by mixed agglutination and absorption-elution techniques. By modifying the methods of Kouvarik et al [1] and Davidsohn [2], Ishiyama et al [3] recently developed a new technique for mixed cell agglutination reaction (MCAR) as follows: 1. Specimens were affixed onto the adhesive side of cellophane tape and then placed on a microscope slide. 2. Anti-A and anti-B agglutinins and Ulex anti-H lectin were used for sensitization. 3. The specimens were thoroughly washed to remove nonreactive agglutinins. 4. A corresponding indicator cell aliquot was added (sandwich method). 5. The slide was reversed and immersed in saline so that the nonreactive cells would dissociate spontaneously. 6. The results were read microscopically.

Author Information

Ishiyama, I
Teikyo University School of Medicine, Tokyo, Japan
Orui, M
Teikyo University School of Medicine, Tokyo, Japan
Ogawa, K
Teikyo University School of Medicine, Tokyo, Japan
Kimura, T
Teikyo University School of Medicine, Tokyo, Japan
Pages: 11
Price: $25.00
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Stock #: JFS10598J
ISSN: 0022-1198
DOI: 10.1520/JFS10598J