Volume 22, Issue 2 (April 1977)
The Determination of Isoantigenic Activity from Latent Fingerprints: Mixed Cell Agglutination Reaction in Forensic Serology
In forensic serology, A-B-H blood group substances have been successfully detected by mixed agglutination and absorption-elution techniques. By modifying the methods of Kouvarik et al  and Davidsohn , Ishiyama et al  recently developed a new technique for mixed cell agglutination reaction (MCAR) as follows: 1. Specimens were affixed onto the adhesive side of cellophane tape and then placed on a microscope slide. 2. Anti-A and anti-B agglutinins and Ulex anti-H lectin were used for sensitization. 3. The specimens were thoroughly washed to remove nonreactive agglutinins. 4. A corresponding indicator cell aliquot was added (sandwich method). 5. The slide was reversed and immersed in saline so that the nonreactive cells would dissociate spontaneously. 6. The results were read microscopically.