(Received 30 August 1974; accepted 3 April 1975)
Published Online: January
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Weigele and co-workers [1,2] reported the structure and synthesis of the reagent 4-phenylspiro [furan-2(3H), 1'-phthalan]-3,3'-dione (fluorescamine), which reacts with substances containing primary amino groups to yield highly fluorescent products. Recently, Undenfriend and co-workers [3–5] published a paper describing the use of fluorescamine in biochemical analysis. This reagent was of interest because of its possible use to improve the detection and differentiation of amphetamine and its relatives in biological samples and solid dosage forms. The main subject of this paper is to present data which clearly demonstrate the use of fluorescamine in forensic toxicological analysis of amphetamine and differentiation from methamphetamine. This reagent also has potential application for analysis of other drugs containing a primary amine group.
Forensic toxicological mass spectroscopist, U.S. Army Criminal Investigation Laboratory-Pacific USACIL-Pacific),
Stock #: JFS10348J