ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 October 1975
Page Count: 5

The Differential Elution of Drugs from XAD-2 Resin
Stolman, A
Chief Toxicologist, Connecticut State Department of Health, Conn.

Pranitis, PAF
Toxicological chemist, Connecticut State Department of Health, Conn.

(Received 14 March 1975; accepted 20 April 1975)

Abstract

In a previously published XAD-2 resin method for the extraction of drugs from biofluids and tissue [1] the elution of the drug(s) from the resin was accomplished with one or more organic solvents. Drugs exhibiting acid, basic, and neutral characteristics were eluted from the column in the same fraction. Preliminary drug screening was usually carried out by thin-layer chromatography (TLC), employing a spray sequence which can achieve detection of almost all the commonly encountered drugs. This procedure of drug detection and identification requires that the analyst be especially competent in utilizing a spray sequence effectively. A preliminary study was conducted to determine whether the drugs eluted differentially from XAD-2 resin. Herein is presented a technique in which drugs are separated physically into acid or neutral, and basic drug fractions.

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Paper ID: JFS10325J
DOI: 10.1520/JFS10325J
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