Volume 20, Issue 4 (October 1975)
The Differential Elution of Drugs from XAD-2 Resin
In a previously published XAD-2 resin method for the extraction of drugs from biofluids and tissue  the elution of the drug(s) from the resin was accomplished with one or more organic solvents. Drugs exhibiting acid, basic, and neutral characteristics were eluted from the column in the same fraction. Preliminary drug screening was usually carried out by thin-layer chromatography (TLC), employing a spray sequence which can achieve detection of almost all the commonly encountered drugs. This procedure of drug detection and identification requires that the analyst be especially competent in utilizing a spray sequence effectively. A preliminary study was conducted to determine whether the drugs eluted differentially from XAD-2 resin. Herein is presented a technique in which drugs are separated physically into acid or neutral, and basic drug fractions.