A Cellulose Acetate Membrane Technique for the Determination of Adenylate Kinase Types in Bloodstains

    Volume 20, Issue 4 (October 1975)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 October 1975

    Page Count: 4


    Saenger, MS
    Forensic serologists, Serology Section, U.S. Army Criminal Investigation Laboratory, Frankfurt am Main,

    Yates, RG
    Forensic serologists, Serology Section, U.S. Army Criminal Investigation Laboratory, Frankfurt am Main,

    (Received 4 March 1975; accepted 17 March 1975)

    Abstract

    The genetically determined isoenzyme blood group system of adenylate kinase (AK) has been demonstrated in lysates of human erythrocytes [1,2] and in bloodstains [3]. The technique employed was horizontal starch gel electrophoresis using either a discontinuous histidine-citrate [1], a phosphate [2], or a succinate [4] buffer system. Since then, electrophoresis on cellulose acetate membrane (CAM) has been introduced as a rapid technique for the determination of AK types in fresh lysates [5,6]. We decided to investigate the use of CAM for determining AK types in bloodstains. In preliminary tests with CAM, we found the discontinuous histidine-citrate buffer system [1,5] gave clear results with lysates, but unsatisfactory results with even fresh bloodstain material. The phosphate buffer [6,7] seemed more promising and this paper describes our evaluation and adaptation of the phosphate system for bloodstain samples.


    Paper ID: JFS10315J

    DOI: 10.1520/JFS10315J

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    Title A Cellulose Acetate Membrane Technique for the Determination of Adenylate Kinase Types in Bloodstains
    Symposium , 0000-00-00
    Committee E30