A Simple, Rapid 125I Radioimmunoassay for the Detection of Barbiturates in Biological Fluids

    Volume 20, Issue 1 (January 1975)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1975

    Page Count: 13


    Grunberg, E
    Associate director of biological research, Hoffman-LaRoche, Inc., Nutley, N.J.

    Heveran, J
    Senior scientist and research group chief, Hoffman-LaRoche, Inc., Nutley, N.J.

    Cleeland, R
    Director, Hoffman-LaRoche, Inc., Nutley, N.J.

    Davis, R
    Senior scientist and research group chief, Hoffman-LaRoche, Inc., Nutley, N.J.

    (Received 25 March 1974; accepted 22 June 1974)

    Abstract

    A radioimmunoassay using a 3H- or 125I-labeled morphine antigen has been shown to be of practical value for the rapid detection of nanogram amounts of morphine and morphine analogs in biological fluids [1–3]. A radioimmunoassay for detection of barbiturates by conjugation of a secobarbital derivative to protein was also developed utilizing 14C-labeled pentobarbital or barbital [4,5]. The present report describes our experiences with a practical radioimmunoassay for barbiturates employing a secobarbital derivative labeled with 125I, as the antigen.


    Paper ID: JFS10239J

    DOI: 10.1520/JFS10239J

    ASTM International
    is a member of CrossRef.

    Author
    Title A Simple, Rapid 125I Radioimmunoassay for the Detection of Barbiturates in Biological Fluids
    Symposium , 0000-00-00
    Committee E30