Journal Published Online: 01 January 1975
Volume 20, Issue 1

A Simple, Rapid I Radioimmunoassay for the Detection of Barbiturates in Biological Fluids

CODEN: JFSCAS

Abstract

A radioimmunoassay using a 3H- or 125I-labeled morphine antigen has been shown to be of practical value for the rapid detection of nanogram amounts of morphine and morphine analogs in biological fluids [1–3]. A radioimmunoassay for detection of barbiturates by conjugation of a secobarbital derivative to protein was also developed utilizing 14C-labeled pentobarbital or barbital [4,5]. The present report describes our experiences with a practical radioimmunoassay for barbiturates employing a secobarbital derivative labeled with 125I, as the antigen.

Author Information

Cleeland, R
Hoffman-LaRoche, Inc., Nutley, N.J.
Davis, R
Hoffman-LaRoche, Inc., Nutley, N.J.
Heveran, J
Hoffman-LaRoche, Inc., Nutley, N.J.
Grunberg, E
Hoffman-LaRoche, Inc., Nutley, N.J.
Pages: 13
Price: $25.00
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Stock #: JFS10239J
ISSN: 0022-1198
DOI: 10.1520/JFS10239J