ISSN: 0022-1198
CODEN: JFSCA
Published Online: 1 January 1975
Page Count: 13

A Simple, Rapid ¹²⁵I Radioimmunoassay for the Detection of Barbiturates in Biological Fluids
Grunberg, E
Associate director of biological research, Hoffman-LaRoche, Inc., N.J.

Heveran, J
Senior scientist and research group chief, Hoffman-LaRoche, Inc., N.J.

Cleeland, R
Director, Hoffman-LaRoche, Inc., N.J.

Davis, R
Senior scientist and research group chief, Hoffman-LaRoche, Inc., N.J.

(Received 25 March 1974; accepted 22 June 1974)

Abstract

A radioimmunoassay using a ³H- or ¹²⁵I-labeled morphine antigen has been shown to be of practical value for the rapid detection of nanogram amounts of morphine and morphine analogs in biological fluids [1–3]. A radioimmunoassay for detection of barbiturates by conjugation of a secobarbital derivative to protein was also developed utilizing ¹⁴C-labeled pentobarbital or barbital [4,5]. The present report describes our experiences with a practical radioimmunoassay for barbiturates employing a secobarbital derivative labeled with ¹²⁵I, as the antigen.

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Paper ID: JFS10239J
DOI: 10.1520/JFS10239J
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