Identification of Flurazepam (Dalmane®) and a Primary Metabolite in Urine by Thin-Layer Chromatography

    Volume 20, Issue 1 (January 1975)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1975

    Page Count: 7


    Manno, BR
    Pharmacologist, Medical Research, Veterans Administration Hospital, and associate professor, Louisiana State Univerisity Medical School in Shreveport, Shreveport, La.

    Manno, JE
    Associate professor, Louisiana State University Medical School i n Shreveport, Shreveport, La.

    Baker, JR
    Medical technologist, Clinical Laboratory, Veterans Administration Hospital, Shreveport, La.

    Hughes, JR
    Chief of Staff, Veterans Administration Hospital, Shreveport, La.

    (Received 8 March 1974; accepted 18 July 1974)

    Abstract

    Flurazepam hydrochloride (Dalmane®) is a relatively new hypnotic agent gaining widespread usage in adults as a “sleeping pill.” Flurazepam is structurally related to the diazebenzapine tranquilizers, diazepam (Valium®) and chlorodiazepoxide hydrochloride (Librium®) It is not classified by Drug Enforcement Administration (DEA) regulations as a drug of abuse; however, any hypnotic, sedative, or tranquilizer which has widespread usage always has the potential for abuse. Until the recent mention by Sturner and Garriott [1] in their article on L-DOPA poisoning, few methods have been available for the detection of flurazepam in blood or urine or both with the exception of those by Swartz et al [2,3] and de Silva and Strojny [4]. A relatively simple, reliable method for the detection of flurazepam in urine could assist in identifying persons abusing or overdosed with the drug. This report describes a simple thin-layer chromatographic (TLC) method for the qualitative identification of flurazepam in urine based upon the presence of flurazepam and a primary urinary metabolite, 7-chloro-1-(2-hydroxyethyl)-5-(2-fluorophenyl)-1,dihydro-2H-1,4-benzodiazepin-2-one (FM).


    Paper ID: JFS10238J

    DOI: 10.1520/JFS10238J

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    Author
    Title Identification of Flurazepam (Dalmane®) and a Primary Metabolite in Urine by Thin-Layer Chromatography
    Symposium , 0000-00-00
    Committee E30