Volume 19, Issue 3 (July 1974)
Hydrolysis of Morphine Glucuronide
In procedures requiring the quantitative analysis for total morphine in body fluids there is a need to liberate the bound drug, and for the hydrolysis of conjugates in urine there are alternative methods of acid or enzyme hydrolysis. However, with the former method questions are raised as to optimum acid concentration and time of hydrolysis, as well as to the relative efficiencies of autoclaving and refluxing; with the latter there are questions of preferred source of enzyme, pH value of the reaction mixture, and incubation temperature. Before the advent of automated analysis for the rapid determination of morphine, a considerable number of tedious and repetitive analyses would have been required to produce acceptable answers, but the use of an AutoAnalyser® manifold has made it a relatively simple matter.