Hydrolysis of Morphine Glucuronide

    Volume 19, Issue 3 (July 1974)

    ISSN: 0022-1198


    Published Online: 1 July 1974

    Page Count: 8

    Fish, F
    Reader in pharmacognosy and forensic science, University of Strathclyde, Glasgow,

    Hayes, TS
    Research assistant in forensic science, Home Office Central Research Establishment, Nr. Reading, Berkshire

    (Received 16 July 1973; accepted 21 November 1973)


    In procedures requiring the quantitative analysis for total morphine in body fluids there is a need to liberate the bound drug, and for the hydrolysis of conjugates in urine there are alternative methods of acid or enzyme hydrolysis. However, with the former method questions are raised as to optimum acid concentration and time of hydrolysis, as well as to the relative efficiencies of autoclaving and refluxing; with the latter there are questions of preferred source of enzyme, pH value of the reaction mixture, and incubation temperature. Before the advent of automated analysis for the rapid determination of morphine, a considerable number of tedious and repetitive analyses would have been required to produce acceptable answers, but the use of an AutoAnalyser® manifold has made it a relatively simple matter.

    Paper ID: JFS10223J

    DOI: 10.1520/JFS10223J

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    Title Hydrolysis of Morphine Glucuronide
    Symposium , 0000-00-00
    Committee E30