Journal Published Online: 01 January 1974
Volume 19, Issue 1

Spermine and Choline Identification by Thin-Layer Chromatography

CODEN: JFSCAS

Abstract

Various methods are available for the detection of prostatic acid phosphatase [1, 2], spermine, choline [3], and spermatozoa. Aspermatic seminal stains are increasing in number due to vasectomies. In these cases location of spermatozoa is not possible. Difficulties are also frequently encountered in the location of spermatozoa even when present in dried seminal stains. This paper sets forth a practical, rapid, inexpensive, and sensitive thin-layer chromatographic method for the detection of spermine and choline. No interferences have been noted from blood, vaginal secretions, saliva, douche powders, urine, or the azo dye (Fast Blue B) for the detection of prostatic acid phosphatase.

Author Information

Hallcock, RR
Iowa Bureau of Criminal Investigation Laboratory, Des Moines, Iowa
Pages: 3
Price: $25.00
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Stock #: JFS10087J
ISSN: 0022-1198
DOI: 10.1520/JFS10087J