(Received 8 February 1973; accepted 28 June 1973)
Published Online: January
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Various methods are available for the detection of prostatic acid phosphatase [1, 2], spermine, choline , and spermatozoa. Aspermatic seminal stains are increasing in number due to vasectomies. In these cases location of spermatozoa is not possible. Difficulties are also frequently encountered in the location of spermatozoa even when present in dried seminal stains. This paper sets forth a practical, rapid, inexpensive, and sensitive thin-layer chromatographic method for the detection of spermine and choline. No interferences have been noted from blood, vaginal secretions, saliva, douche powders, urine, or the azo dye (Fast Blue B) for the detection of prostatic acid phosphatase.
Chemist, Iowa Bureau of Criminal Investigation Laboratory, Des Moines, Iowa
Stock #: JFS10087J