Spermine and Choline Identification by Thin-Layer Chromatography

    Volume 19, Issue 1 (January 1974)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Published Online: 1 January 1974

    Page Count: 3


    Hallcock, RR
    Chemist, Iowa Bureau of Criminal Investigation Laboratory, Des Moines, Iowa

    (Received 8 February 1973; accepted 28 June 1973)

    Abstract

    Various methods are available for the detection of prostatic acid phosphatase [1, 2], spermine, choline [3], and spermatozoa. Aspermatic seminal stains are increasing in number due to vasectomies. In these cases location of spermatozoa is not possible. Difficulties are also frequently encountered in the location of spermatozoa even when present in dried seminal stains. This paper sets forth a practical, rapid, inexpensive, and sensitive thin-layer chromatographic method for the detection of spermine and choline. No interferences have been noted from blood, vaginal secretions, saliva, douche powders, urine, or the azo dye (Fast Blue B) for the detection of prostatic acid phosphatase.


    Paper ID: JFS10087J

    DOI: 10.1520/JFS10087J

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    Title Spermine and Choline Identification by Thin-Layer Chromatography
    Symposium , 0000-00-00
    Committee E30