Analysis of non-suspect samples lacking visually identifiable sperm using a Y-STR 10-Plex

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Analysis of non-suspect samples lacking visually identifiable sperm using a Y-STR 10-Plex Johnson CL, Giles RC, Warren JH, Floyd JI, Staub RW
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Abstract
Y-STRs are valuable in the investigation of sexual assaults in which autosomal STR genotype interpretation is challenging. To detect male DNA from compromised sexual assault evidence, 45 non-suspect samples were differentially extracted and analyzed with 10 Y-STRs. These samples were positive for the presence of human seminal fluid, but were negative for spermatozoa by microscopic examination. Y-STR data were obtained in ~86.2% of the epithelial or sperm fractions. On samples yielding incomplete profiles, results were obtained on an average of 5 loci per sample. The inability to obtain results may be due to insufficient amplifiable male DNA, PCR inhibition, or unfounded accusations of sexual assault. This study indicates that it is possible to obtain a male STR profile even in the absence of visually identifiable spermatozoa. Furthermore, Y-STR loci should become components of CODIS if they are to be used in solving non-suspect sexual assaults.

Keywords:
DNA typing, DYS19, DYS391, DYS392, DYS436, DYS437, DYS438, DYS439, DYS460, forensic science, sexual assault, short tandem repeaats, short tandem repeat, Y-chromosome, Y-GATA-H4
ISSN: 0022-1198
Paper ID: JFS2005052
DOI: 10.1520/JFS2005052

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Author Johnson CL, Giles RC, Warren JH, Floyd JI, Staub RW Title Analysis of non-suspect samples lacking visually identifiable sperm using a Y-STR 10-Plex Symposium , Committee on

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