An <I>Alu</I>-based, MCG Eclipse™ Real-time PCR method for quantitation of human DNA in forensic samples

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*An Alu-based, MCG Eclipse™ Real-time PCR method for quantitation of human DNA in forensic samples* Nicklas JA, Buel E
Download this paper for $25 PDF (320K) View License Agreement Source: JFS (August 2005)

Abstract
The forensic community needs quick, reliable methods to quantitate human DNA in crime scene samples to replace the laborious and imprecise slot blot method. A real-time PCR based method has the possibility of allowing development of a faster and more quantitative assay. Alu sequences are primate-specific and are found in many copies in the human genome, making these sequences an excellent target or marker for human DNA. This paper describes the development of a real-time Alu sequence-based assay using MGB Eclipse™ primers and probes. The advantages of this assay are simplicity, speed, less hands-on-time and automated quantitation, as well as a large dynamic range (128 ng/mL to 0.5 pg/mL).

Keywords:
DNA quantification, DNA quantitation, forensic science, human DNA, polymerase chain reaction, real-time
ISSN: 0022-1198
Paper ID: JFS2004514
DOI: 10.1520/JFS2004514

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Author Nicklas JA, Buel E Title An Alu-based, MCG Eclipse™ Real-time PCR method for quantitation of human DNA in forensic samples Symposium , Committee on

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