mtDNA investigations after differential lysis

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mtDNA investigations after differential lysis Anslinger KD, Bayer BR, Rolf B, Eisenmenger W
Download this paper for $25 PDF (136K) View License Agreement Source: JFS (March 2005)

Abstract
In order to gain information regarding the ethnic origin of an unknown offender in a murder case it was necessary to sequence the HV1 and 2 regions of the mitochondrial DNA (mtDNA). The only evidentiary material that could be linked to the suspect was DNA, extracted from spermatozoa after differential lysis. The observed mtDNA sequences were identical to the sequences of the victim. Therefore, we had to check if this was a coincidence or the result of a technical limitation of the testing procedure. Subsequently, we performed a systematic study. In cases with complete separation of sperm and female cells it wasn't possible to obtain a mtDNA sequence for the sperm fraction. This phenomena is based on the loss of the sperm's flagellum and mid-piece during the first lysis step and a concomitant loss of the sperm's mitochondria. In our murder case, a minor carry-over of female cells to the sperm fraction was responsible for the sequencing result.

Keywords:
differential lysis, forensic science, mtDNA, sperm
ISSN: 0022-1198
Paper ID: JFS2004217
DOI: 10.1520/JFS2004217

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Author Anslinger KD, Bayer BR, Rolf B, Eisenmenger W Title mtDNA investigations after differential lysis Symposium , Committee on

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