Forensic casework analysis using the HVI/HVII mtDNA linear array assay

Publish With ASTM

Book Guide
Journals

Site Map

Online Support

Site Search

Subscriptions/Pricing

Digital Library/Journal of Forensic Sciences/

Forensic casework analysis using the HVI/HVII mtDNA linear array assay Divne AM, Nilsson M, Calloway C, Reynolds R, Erlich H, Allen M
Download this paper for $25 PDF (224K) View License Agreement Source: JFS (March 2005)

Abstract
The mitochondrial hypervariable regions I and II have proven to be a useful target for analysis of forensic materials, in which the amount of DNA is limited or highly degraded. Conventional mitochondrial DNA (mtDNA) sequencing can be time-consuming and expensive, limitations that can be minimized using a faster and less expensive typing assay. We have evaluated the exclusion capacity of the linear array mtDNA HVI/HVII region-sequence typing assay (Roche Applied Science) in 16 forensic cases comprising 90 samples. Using the HVI/HVII mtDNA linear array, 56% of the samples were excluded and thus less than half of the samples require further sequencing due to a match or inconclusive results. Of all the samples that were excluded by sequence analysis, 79% could be excluded using the HVI/HVII linear array alone. Using the HVI/HVII mtDNA linear array assay, we demonstrate the potential to decrease sequencing efforts substantially and thereby reduce the cost and the turn-around time in casework analysis.

Keywords:
D-loop, forensic science, immobilized SSO probe assay, linear array mtDNA HVI/HVII region-sequence typing, mtDNA
ISSN: 0022-1198
Paper ID: JFS2004505
DOI: 10.1520/JFS2004505

ASTM International is a member of CrossRef.
Author Divne AM, Nilsson M, Calloway C, Reynolds R, Erlich H, Allen M Title Forensic casework analysis using the HVI/HVII mtDNA linear array assay Symposium , Committee on

Site Map | Online Support | Contact | Privacy Policy | IP Policy