Rapid determination of carbohydrates in heroin drug seizures using capillary electrophoresis with short-end injection

Publish With ASTM

Book Guide
Journals

Site Map

Online Support

Site Search

Subscriptions/Pricing

Digital Library/Journal of Forensic Sciences/

Rapid determination of carbohydrates in heroin drug seizures using capillary electrophoresis with short-end injection Anastos N, Barnett NW, Lewis SW, Pearson JR, Kirkbride KP
Download this paper for $25 PDF (87K) View License Agreement Source: JFS (July 2005)

Abstract
A simple and rapid method for the analysis of carbohydrates in heroin samples by capillary electrophoresis utilizing a borate complexation method is described. Separations were performed using an uncoated fused silica capillary, 50 cm × 50 mm I.D. × 360 mm O.D. with an effective separation length of 9 cm. The system was run at 60°C with an applied voltage of -8 kilovolts. Injection of each sample was for 1 sec at -50 mbar. UV detection was employed with the wavelength set at 195 nm. The background electrolyte consisted of 65 mM borate, pH 12.0. Samples and standards were prepared in the run buffer containing 2 mg/mL of mannose as an internal standard. Under these conditions a test mixture containing glucose, sucrose, lactose, mannitol and mannose as an internal standard was resolved within 5 min. The method was used to determine the concentration of carbohydrates in heroin seizure samples and synthetic heroin samples. The results were in good agreement with the reported values.

Keywords:
capillary electrophoresis, forensic science, heroin seizures, short-end injection
ISSN: 0022-1198
Paper ID: JFS2004549
DOI: 10.1520/JFS2004549

ASTM International is a member of CrossRef.
Author Anastos N, Barnett NW, Lewis SW, Pearson JR, Kirkbride KP Title Rapid determination of carbohydrates in heroin drug seizures using capillary electrophoresis with short-end injection Symposium , Committee on

Site Map | Online Support | Contact | Privacy Policy | IP Policy