A PCR multiplex and database for forensic DNA identification of dogs

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A PCR multiplex and database for forensic DNA identification of dogs Halverson J, Basten C, Halverson J, Basten C
Download this paper for $25 PDF (576K) View License Agreement Source: JFS (January 2005)

Abstract
Animal-derived trace evidence is a common finding at crime scenes and may provide an important link between victim(s) and suspect(s). A database of 558 dogs of pure and mixed breeds is described and analyzed with two PCR multiplexes of 17 microsatellites. Summary statistics (number of alleles, expected and observed heterozygosity and power of exclusion) are compared between breeds. Marked population substructure in dog breeds indicates significant inbreeding, and the use of a conservative **theta** value is recommended in likelihood calculations for determining the significance of a DNA match. Evidence is presented that the informativeness of the canine microsatellites, despite inbreeding, is comparable to the human CODIS loci. Two cases utilizing canine DNA typing, State of Washington v. Kenneth Leuluaialii and George Tuilefano and Crown v. Daniel McGowan, illustrate the potential of canine microsatellite markers for forensic investigations.

Keywords:
canine, CODIS, database, DNA typing, forensic science, likelihood ratio, microsatellites, polymerase chain reaction, STR
ISSN: 0022-1198
Paper ID: JFS2004207
DOI: 10.1520/JFS2004207

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Author Halverson J, Basten C, Halverson J, Basten C Title A PCR multiplex and database for forensic DNA identification of dogs Symposium , Committee on

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