ASTM WK44955

    New Test Method for Determination of the viable aerobic microbial content of fuels and associated water - Thixotropic Gel Culture Method.

    (What is a Work Item?)

    Developed by Subcommittee: D02.14 | Committee D02 | Contact Staff Manager


    1. Scope

    1. Scope 1.1 This standard describes a procedure that can be used in the field or in a laboratory to quantify culturable, viable aerobic microorganisms present as contaminants in liquid fuels and fuel-associated water. The test method may also be used to analyze other hydrocarbon, aqueous and non-aqueous liquids and products but these applications fall outside the scope of this method. 1.2 The test can quantitatively assess viable aerobic microbial content as microbial colony forming units (CFU). 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For a specific hazard statement, see Section 9.

    The only current culture method (ASTM D6974) is a laboratory protocol. Recognizing the perishability of fuel samples intended for microbiological testing, it would be of considerable value to have a simple, reliable field method for determining the presence of culturable microbial contamination in fuels and fuel associated waters. The method will be suitable for use by anyone performing condition monitoring or microbial contamination diagnosis of fuel storage systems.


    bacteria; biocontamination; colony forming units (CFU); fuel; fungi; microbial contamination; microbial growth; microbiology; MicrobMonitor2; thixotropic gel culture; viable aerobic microorganisms.

    The title and scope are in draft form and are under development within this ASTM Committee.

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    Work Item Status

    Date Initiated:

    Technical Contact:
    Graham Hill


    D02.14 (14-05)

    Withdrawn From Balloting


    D02 (14-07)

    Ballot Item Approved as D7978-2014 and Pending Publication