ASTM WK43261

    New Practice for Determining the Relative Spectral Correction Factors for the Emission Signal of Fluorescence Spectrometers

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    Developed by Subcommittee: E13.01 | Committee E13 | Contact Staff Manager


    1. Scope

    This practice describes three methods for determining the relative spectral correction factors for grating-based fluorescence spectrometers in the ultraviolet-visible spectral range. These methods are intended for instruments with a 0/90 transmitting sample geometry. Each method uses different types of transfer standards, including 1) a calibrated light source (CS), 2) a calibrated detector (CD) and a calibrated diffuse reflector (CR), and 3) certified reference materials (CRMs). The wavelength region covered by the different methods ranges from 250 nm to 830 nm with some methods having a broader range than others. Extending these methods to the near infrared (NIR) beyond 830 nm will be discussed briefly, when appropriate. These methods were designed for scanning fluorescence spectrometers with a single channel detector, but can also be used with a multichannel detector, such as a diode array or a CCD.


    Emission Signal; Fluorescence; Spectral

    The title and scope are in draft form and are under development within this ASTM Committee.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Paul De Rose


    E13 (15-02)

    Ballot Item Approved as E3029-2015 and Pending Publication