ASTM WK42958

    New Test Method for Assessing the Ability of Pre-Wetted Towelettes to Remove and Transfer Bacterial Contamination on Hard, Non-Porous Environmental Surfaces Using the Wiperator

    (What is a Work Item?)

    Developed by Subcommittee: E35.15 | Committee E35 | Contact Staff Manager


    1. Scope

    1.1 This test method is designed for use with a mechanized device (the Wiperator, Appendix X1) to test pre-wetted towelettes. 1.2 Two species of vegetative bacteria, one Gram-positive coccus and one Gram-negative bacillus, representing important nosocomial pathogens, are used to contaminate separately disks of magnetized and brushed stainless steel to test the towelettes for their relative ability to: 1.2.1 Decontaminate nonporous environmental surfaces experimentally contaminated with vegetative bacteria and 1.2.2 Transfer any acquired bacterial contamination on the towelettes to clean surfaces. 1.3 This test method is not meant for use with towelettes for decontamination of skin. 1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses, if any, are for information only. 1.5 This test method should be performed by persons with training in microbiology in facilities designed and equipped for work with infectious agents at the appropriate biosafety level. 1.6 It is the responsibility of the investigator to determine whether good laboratory practice (GLP) regulations are required and to follow them where appropriate. 1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    There is increasing popularity of disinfectant-soaked wipes for decontamination of nonporous environmental surfaces in healthcare and other settings. However, any testing of such wipes does not properly reflect their field use. The proposed standard would be of interest to manufacturers, end users, government regulators, as well as researchers.


    Acinetobacter baumannii; decontamination; disinfection; environmental surfaces; infection control;; microbial transfer; nosocomial pathogens; Staphylococcus aureus; towelette; Wiperator; wiping

    The title and scope are in draft form and are under development within this ASTM Committee.

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    Work Item Status

    Date Initiated:

    Technical Contact:
    Syed Sattar


    E35 (15-01)

    Ballot Item Approved as E2967-2015 and Pending Publication