ASTM WK42082

    Revision of D5392 - 93(2006) Standard Test Method for Isolation and Enumeration ofEscherichia Coli in Water by the Two-Step Membrane Filter Procedure

    (What is a Work Item?)

    Active Standard: D5392 - 14

    Developed by Subcommittee: D19.24 | Committee D19 | Contact Staff Manager


    1. Rationale

    D5392 was initially approved in 1993, whilst the EPA 1603, modified m-TEC method was released in 2002 as an alternative to the Two Step Membrane Filtration Method. The commenter indicated that the modified method should be incorporated into this Standard. This modified method to become an ASTM Standard, would require submission as a proposed method and complete the requirements for a new method. It cannot be incorporated into the existing standard. This negative vote for incorporation of the modified method is not persuasive. However, the commenter is correct that three of the reagents indicated in this Standard for confirmation does not have the ingredients or procedure in section 8. This will be added to the Standard and will be reviewed at the next D19-24 sub-committee meeting.


    ambient waters; Escherichia coli; fecal pollution; mTEC agar; recreational waters; two-step membrane filter method; wastewaters;
    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Gil Dichter


    D19 (13-02)

    Ballot Item Approved as D5392-2014 and Pending Publication