ASTM WK40215

    New Test Method for Applying Aerosolized Threat Representative Viruses On Inanimate Surfaces

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    Developed by Subcommittee: E35.15 | Committee E35 | Contact Staff Manager


    1. Scope

    The method will provide guidance for applying aerosolized Helicoverpa zea single capsid nucleopolyhedrovirus (HzSNPV) virus onto surfaces ; subsequent decontamination testing of the virus or evaluation on germicidal surfaces will follow. HZSNPV is a simulant virus for Variola. Methods for fluidizing, stabilizing the virus, and preparing threat representative aerosol conditions will be provided.

    The method by which microorganisms are applied to a surface will impact the ability to decontaminate them for some decontamination processes. This standard is focused on mimicking methods of contamination that would be likely during a viral biological attack. It also proposes the use of a novel BSL-1 simulant virus, which will simply its use. The method will provide a means to test decontamination methods that may be needed to be used following a malicious bioaerosol attack.


    Aerosol, Virus, Variola, Helicoverpa, Decontamination

    The title and scope are in draft form and are under development within this ASTM Committee.

    Citing ASTM Standards
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    Work Item Status

    Date Initiated:

    Technical Contact:
    Brian Heimbuch

    Draft Under Development