ASTM WK32823

    New Test Method for In Vitro Production of Clostridium Difficile Spores

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    Developed by Subcommittee: E35.15 | Committee E35 | Contact Staff Manager



    WK32823

    1. Scope

    1.1 This test method is designed to propagate spores of Clostridium difficile using a broth culture method. 1.2 It is the responsibility of the user of this test method to determine whether good laboratory practices are required and follow when appropriate. 1.3 This test method should only be performed by those trained in microbiological techniques. 1.4 Units--The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    This proposed test method would be used by microbiology laboratories to propagate spore crops of C. difficile for use in a variety of efficacy tests. This proposed standard is a simple, easy-to-perform test method that will sporulate several different strains of C. difficile.


    Keywords

    Clostridium; C. difficile; spores; propagation; sporulation; spore crop

    The title and scope are in draft form and are under development within this ASTM Committee.

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    Work Item Status

    Date Initiated:
    04-04-2011

    Technical Contact:
    Lisa Hellickson

    Item:
    003

    Ballot:
    E35.15 (13-05)

    Status:
    Negative Votes Need Resolution