1.1 The investigation of fundamental processes in life science relies on the fast, sensitive, reliable and reproducible detection of the interplay of biomolecules with one another or with other species. Fluorescent techniques fulfill these goals. The potential of a fluorescent detection or imaging method is determined, to a great extent, by the physicochemical properties of the chromophore used. A quantitative study of cells using fluorescent techniques require well characterized fluorescent materials. This guide describes test methods suitable for characterizing such fluorophores and highlights specific measurement protocols that should be followed in their characterization. 1.2 Some of the techniques or protocols described are also applicable to wider classes of materials including e.g.
Keywordsfluorophore, fluorescent material, fluorescence microscopy, biological cells, protein, assay:
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Draft Under Development