1.1 This document describes how to conduct in vitro osteoblast differentiation assays in which human progenitor cells are cultured under conditions that promote differentiation to mature osteoblasts. 1.2 This document describes the roles of various osteogenic supplements to cell culture medium that can be used in vitro to differentiate progenitor cells into mature osteoblasts. 1.3 This document provides information about the concentrations of osteogenic supplements to use to avoid precipitation of non-physiological mineral deposits that are independent of the presence of mature osteoblasts and are not correlated to osteoblastic gene expression. 1.4 This document only applies to the culture of human progenitors, and not cells from other species.
The purpose of the standard is to standardize in vitro mineralization conditions and the use of controls since an excess of calcium and phosphate in the cell culture medium can lead to mineral deposition not associated with osteoblasts and may lead to misinterpretation of the assay.
Keywordsosteoblasts, bone, in vitro mineralization
The title and scope are in draft form and are under development within this ASTM Committee.Back to Top