ASTM WK28565

    Work Item: ASTM WK28565 - New Practice for the Collection of Culturable Airborne Fungi on Agar Plates by Inertial Impaction Systems

    Developed by Subcommittee: D22.08 | Committee D22 | Contact Staff Manager



    1. Scope

    The purpose of this standard practice is to describe procedures for the collection of culturable airborne fungal spores and/or fragments on agar plates using inertial impaction sampling techniques. This practice does not include collection of culturable fungi by devices not using agar plates. This standard practice presumes that the user has a fundamental understanding of field investigative techniques related to the scientific process, and sampling plan development and implementation. It is important to establish the related hypothesis to be tested and the supporting analytical methodology needed in order to identify the sampling media to be used and the laboratory conditions for analysis. This standard practice does not provide the user sufficient information to allow for interpretation of the analytical results from sample collection. It is the users responsibility to seek or obtain the information and knowledge necessary to interpret the sample results reported by the laboratory.

    This standard practice is intended for the collection of airborne particles on agar plates using inertial impaction for the purpose of culturing fungi. This standard practice is valuable when species level identification and/or quantity of culturable aerosolized fungi are important factors for the indoor air quality investigation. This practice can also be used to collect air samples for culturing bacteria. This standard is intended to provide indoor environment investigators with a basic understanding of the equipment, materials and instructions necessary to effectively collect air samples on agar plates using an inertial impactor. This standard is intended to minimize systematic sampling variations between different data sets.


    Keywords

    Agar Plate; Bioaerosol; Calibration; Collection Efficiency; Colonies; Colony Forming Units; Culturable Fungi; Fungi; Fungus; Hyphae; Impactor; Indoor Air Quality; Inertial Impaction Sampler; Mold; Mould; Sampling; Spore

    The title and scope are in draft form and are under development within this ASTM Committee.

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