1.Scope 1.1 This method covers a bioassay measurement of biochemical oxygen demand (BOD) of surface waters, treated and untreated wastewater, and industrial wastewater, including distinguishing Total BOD (TBOD5) from oxygen demand from carbonaceous oxidation only (CBOD5), and from oxygen demand from oxidation of nitrogen, only (NBOD5). Such oxygen demand is exerted by the following types of materials: 1.1.1 Carbonaceous organic material usable as a source of food by aerobic organisms. 1.1.2 Oxidizable nitrogen derived from nitrites, ammonia, and organic nitrogen compounds which serve as food for specific bacteria (for example, nitrosomonas and nitrobacter). 1.1.3 Certain chemically oxidizable materials (for example, ferrous iron, sulfides, sulfites, etc.) which will react with dissolved oxygen. 1.2 Because the BOD test measures only a portion of the total oxygen that would be utilized for complete microbial metabolism of oxidizible matter, it may be necessary to perform a longer-term oxygen demand test (see Ultimate BOD, DXXXX) where an assessment of the total demand is required. The BOD test contains provisions to assure that there is sufficient active bio-mass present to minimize any lag in oxygen demand exerted during the test. 1.3 Dissolved oxygen determinations made as part of this Standard are performed as described in D8882 methods B (membrane probes) or C (Luminescence Probes). 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
The title and scope are in draft form and are under development within this ASTM Committee.
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