Carbohydrates by HPLC


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    1. Scope

    This method is for determining saccharides in aqueous solutions by high performance liquid chromatography (HPLC). Specific saccharides typically quantitated are tetrasaccharides (DP4+) and unresolved higher polysaccharides; maltotriose including other unresolved trisaccharides (DP3); maltose, maltulose plus other unresolved disaccharides; glucose; mannose plus sorbose; fructose; and psicose. The lower limit of quantitation for an individual component in the aqueous solution is typically 0.01 mass-%.

    Citing ASTM Standards
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