ASTM E1259-05

    Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C


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    1. Scope

    1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D 396, D 910, D 975, D 1655, D 2069, D 2880, D 3699, D 4814, D 6227, and D 6751), system deterioration, or both.

    1.2 Knowledge of microbiological techniques is required for these procedures.

    1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.

    1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


    2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

    ASTM Standards

    D396 Specification for Fuel Oils

    D910 Specification for Leaded Aviation Gasolines

    D975 Specification for Diesel Fuel Oils

    D1655 Specification for Aviation Turbine Fuels

    D2069 Specification for Marine Fuels

    D2880 Specification for Gas Turbine Fuel Oils

    D3699 Specification for Kerosine

    D4814 Specification for Automotive Spark-Ignition Engine Fuel

    D5465 Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

    D6227 Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component

    D6293 Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic(O-PONA) Hydrocarbon Types in Low-Olefin Spark Ignition Engine Fuels by Gas Chromatography

    D6469 Guide for Microbial Contamination in Fuels and Fuel Systems

    D6729 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High Resolution Gas Chromatography

    D6733 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High Resolution Gas Chromatography

    D6751 Specification for Biodiesel Fuel Blend Stock (B100) for Middle Distillate Fuels

    D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels--Filtration and Culture Procedures

    D7463 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water

    D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing

    D7467 Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)

    D7687 Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures, and Fuel-Associated Water with Sample Concentration by Filtration

    D7978 Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water--Thixotropic Gel Culture Method

    E1259

    E1326 Guide for Evaluating Non-culture Microbiological Tests

    Federal Standards

    40 CFR Part 152 Pesticide Registration and Classification Procedures

    40 CFR Part 79 Fuels and Fuel Additives Registration Regulations Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401.


    UNSPSC Code

    UNSPSC Code 51102700(Antiseptics); 47131803(Household disinfectants)


    Referencing This Standard
    Link Here
    Link to Active (This link will always route to the current Active version of the standard.)

    DOI: 10.1520/E1259-05

    ASTM International is a member of CrossRef.


    Citation Format

    ASTM E1259-05, Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390°C, ASTM International, West Conshohocken, PA, 2005, www.astm.org

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